Diversity and potential pathogenicity of microscopic fungi in the Archaeological Museum Park of Tunja at the Pedagogical and Technological University of Colombia

Authors

  • Ana Yervid Rodríguez Sáenz Grupo de investigación atención primaria en salud, Facultad Ciencias de la Salud, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Calle 24 No 5-63, Antiguo Hospital San Rafael, Colombia https://orcid.org/0000-0003-2904-7016
  • Bernardo Meléndez Álvarez Grupo de investigación atención primaria en salud, Facultad Ciencias de la Salud, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Calle 24 No 5-63, Antiguo Hospital San Rafael, Colombia https://orcid.org/0000-0002-7277-5062
  • Pedro María Argüello García Universidad Pedagógica y Tecnológica de Colombia (UPTC), Colombia https://orcid.org/0000-0003-3570-2283

DOI:

https://doi.org/10.14568/cp39647

Keywords:

Fungal identification, Biodeterioration, Fungal diversity, Museum conservation, Pathogenic risk

Abstract

This study assessed the diversity of microscopic fungi in two areas of the Archaeological Museum Park of Tunja, (Universidad Pedagógica y Tecnológica de Colombia), aiming to anlyze their potential impact on human health and their capacity to cause biodeterioration of heritage materials. A total of 50 samples were collected from air, surfaces, bioanthropological remains, and ceramic objects. These samples were cultured on Sabouraud agar with chloramphenicol (0,05 g/L) and incubated at a temperature of 25 °C. Macroscopic and microscopic analyses identified 20 genera of fungi and yeasts, with Penicillium (26,9 %), Cladosporium (15,6 %), Fusarium (14,4 %), and Mucor (5,4 %) being the most prevalent. These genera have been reported in the literature for their association with both infectious processes and material degradation. The findings indicate a dual potential risk, sanitary and conservation-related, highlighting the need to implement monitoring and environmental control strategies to protect both public health and culture heritage.

Downloads

Download data is not yet available.

References

1. Pradilla, H.; Villate, G.; Ortiz, F., ‘Arqueología del cercado grande de los santuarios’, Boletín del Museo del Oro (1993) 21-147.

2. Argüello, P., Tunja Prehispánica, UPTC, Tunja (2023), https://doi.org/10.19053/9789586607940.

3. Mallo, A. C.; Elíades, L. A.; Nitiu, D. S.; Saparrat, M. C. N., ‘Fungal monitoring of the indoor air of the Museo de La Plata Herbarium, Argentina’, Revista Iberoamericana de Micología 34(2) (2017) 99-105, https://doi.org/10.1016/j.riam.2016.05.003.

4. Kour, D.; Khan, S. S.; Gusain, M.; Bassi, A.; Kaur, T.; Kataria, A.;Kaur, S.; Kour, H., ‘Airborne fungal communities: diversity, health impacts, and potential AI applications in aeromycology’, Aerobiology 3(4) (2025) 10, https://doi.org/10.3390/aerobiology3040010.

5. Fashola, M. O.; Ajilogba, C. F.; Aremu, B. R.; Babalola, O. O., ‘Airborne fungi and mycotoxins’, in Aeromicrobiology, Elsevier, London (2023) 147-175, https://doi.org/10.1016/C2021-0-01860-5.

6. Lu, Y.; Wang, X.; Almeida, L. C. S. D. S.; Pecoraro, L., ‘Environmental factors affecting diversity, structure, and temporal variation of airborne fungal communities in a research and teaching building of Tianjin University, China’, Journal of Fungi 8(5) (2022) 431, https://doi.org/10.3390/jof8050431.

7. Sterflinger, K., ‘Fungi: their role in deterioration of cultural heritage’, Fungal Biology Reviews 24(1-2) (2010) 47-55, https://doi.org/10.1016/j.fbr.2010.03.003.

8. Farro-Barbaran, R.; Ramos-Iturregui, W. M.; Iglesias-Osores, S.; Carreño-Farfán, C., ‘Aislamiento e identificación de microorganismos ambientales del museo Tumbas Reales de Sipán’, Revista UDCA Actualidad & Divulgación Científica 24(2) (2021) 1-11.

9. Mircea, C.; Vulpoi, A.; Rusu, I.; Radu, C.; Pârvu, M.; Kelemen, B., ‘Exploring post‐excavation degradation potential of fungal communities associated with archaeological human remains’, Archaeometry 61(3) (2019) 750-763, https://doi.org/10.1111/arcm.12438.

10. Koestler, R. J., ‘Biodeterioration in museum collections’, in Met Objectives, ed. D. Schorsch & M. Manuels, The Sherman Fairchild Center for Objects Conservation, New York (2002), https://repository.si.edu/items/d1e759f7-627d-4421-8d5d-ac09ff2cf1c6 (acceso en 2026-05-22).

11. Carrillo Chavez, L. M.; Arias Bermúdez, N.; Nieto Venegas, M. J.; Pinto Sanchez, N. R.; Patiño, M. C., ‘Study of biodeterioration and evaluation of proteolytic activity caused by microorganisms in specimens of a zoological collection in Colombia’, Conservar Património 45 (2023) 7-20, https://doi.org/10.14568/cp24895.

12. Kakakhel, M. A.; Wu, F.; Gu, J.-D.; Feng, H.; Shah, K.; Wang, W., ‘Controlling biodeterioration of cultural heritage objects with biocides: a review’, International Biodeterioration & Biodegradation 143 (2019) 104721, https://doi.org/10.1016/j.ibiod.2019.104721.

13. Urzì, C.; Leo, F. D., 'Biodeterioration of cultural heritage in Italy: state of art', in European Research on Cultural Heritage. State of Art Studies, vol. 2, ed. D. Milos, Academy of Sciences in The Czech Republic, Praga (2004) 465-471.

14. Pinheiro, A. C.; Sequeira, S. O.; Macedo, M. F., ‘Fungi in archives, libraries, and museums: a review on paper conservation and human health’, Critical Reviews in Microbiology 45(5-6) (2019) 686-700, https://doi.org/10.1080/1040841X.2019.1690420.

15. Wiszniewska, M.; Walusiak-Skorupa, J.; Gutarowska, B.; Krakowiak, A.; Pałczyński, C., ‘Is the risk of allergic hypersensitivity to fungi increased by indoor exposure to moulds?’, International Journal of Occupational Medicine and Environmental Health 22(4) (2009) 343-354, https://doi.org/10.2478/v10001-009-0030-7.

16. Scarlat, I.; Haiducu, M.; Stepa, R., ‘Air contamination with fungals in museum’, ACTA Universitatis Cibiniensis 66(1) (2015) 165-168, https://doi.org/10.1515/aucts-2015-0047.

17. Zielińska-Jankiewicz, K.; Kozajda, A.; Piotrowska, M.; Szadkowska-Stańczyk, I., 'Microbiological contamination with moulds in work environment in libraries and archive storage facilities', Annals of agricultural and environmental medicine 15(1) (2008)71-78.

18. Trovão, J.; Portugal, A.; Soares, F.; Paiva, D. S.; Mesquita, N.; Coelho, C.; Pinheiro, A. C.; Catarino, L.; Gil, F.; Tiago, I., ‘Fungal diversity and distribution across distinct biodeterioration phenomena in limestone walls of the old cathedral of Coimbra, UNESCO World Heritage Site’, International Biodeterioration & Biodegradation 142 (2019) 91-102, https://doi.org/10.1016/j.ibiod.2019.05.008.

19. Cepeda, R.; Luque, L.; Ramirez, D.; Franco, P.; Fabra, M., ‘Monitoreo de hongos ambientales en laboratorios y reservas patrimoniales bioarqueologicas’, Boletín Micológico 34(2) (2019) 33-49, https://doi.org/10.22370/bolmicol.2019.34.2.1909.

20. Micali, O.; Montacutelli, R.; Tarsitani, G., ‘Pathogenic microorganisms and situations of risk to man’, in Cultural heritage and aerobiology: methods and measurement techniques for biodeterioration monitoring, eds. P. Mandrioli, G. Caneva y C. Sabbioni, Springer, Dordrecht (2003) 31-43.

21. Guiamet, P.; Borrego, S.; Lavin, P.; Perdomo, I.; de Saravia, S. G., ‘Biofouling and biodeterioration in materials stored at the Historical Archive of the Museum of La Plata, Argentine and at the National Archive of the Republic of Cuba’, Colloids and Surfaces B: Biointerfaces 85(2) (2011) 229-234, https://doi.org/10.1016/j.colsurfb.2011.02.031.

22. Pérez, H.; Sánchez, V. L., ‘Propuesta de diseño de monitoreo ambiental microbiológico para diagnóstico de niveles de contaminación en áreas de procesamiento aséptico’, ICIDCA. Sobre los Derivados de la Caña de Azúcar 44(3) (2010) 7-14.

23. López-Aranda. B. G., ‘Caracterización microbiológica de los almacenes del museo Tumbas Reales de Sipán en Lambayeque, Perú’, La zaranda de ideas 18(2) (2020) 136-145.

24. Bogomolova, E.; Kirtsideli, I., ‘Airborne fungi in four stations of the St. Petersburg Underground railway system’, International Biodeterioration & Biodegradation 63(2) (2009) 156-160, https://doi.org/10.1016/j.ibiod.2008.05.008.

25. Larone, D. H., Medically important fungi: a guide to identification, 2ª ed., American Society for Microbiology, Washington, D.C (1993)

26. de Hoog, G.; Guarro, J.; Gené, J.; Figueras, M. J., Atlas of clinical fungi, CABI Digital Library (2000), https://www.cabidigitallibrary.org/doi/full/10.5555/20013005530.

27. Carvalho, H. L. da S. P., Biodeterioration in cultural assets: fungal contamination assessment in art objects and documents, tesis doctoral, Departamento de Ciencias da Vida, Universidade de Coimbra, Coimbra (2018).

28. R Core Team, R: A Language and environment for statistical computing, R Foundation for Statistical Computing, Vienna (2023), https://www.R-project.org/ (acceso en 2026-05-22).

29. Oksanen, J; Simpson, G. L.; Guillaume Blanchet, F.; Kindt, R.; Legendre, P.; Minchin, p. R.; O’Hara, R. B.; Solymos, P.; Stevens, M. H. H.; Szoecs, E.; Wagner, H.;. et al., ‘Ordination methods, diversity analysis and other functions for community and vegetation ecologists’, in vegan: Community Ecology Package (2004), https://CRAN.R-project.org/package=vegan (acceso en 2026-05-22).

30. Magurran, A. E., Measuring Biological Diversity, Blackwell Publishing, Oxford (2004).

31. García, J. C. R., ‘Microbiología aplicada: una herramienta parala conservación del Patrimonio Cultural’, Conservar Património 24 (2016) 23-36, https://doi.org/10.14568/cp2015007.

32. Toyoda, A.; Shibata, Y.; Matsuo, Y.; Terada, K.; Sugimoto, H.; Higashi, K.; Mori, H.; Ikeuchi, A.;Ito, M.; Kurokawa, K.; Katahira, S., ‘Diversity and compositional differences of the airborne microbiome in a biophilic indoor environment’, Scientific Reports 13(1) (2023) 8179, https://doi.org/10.1038/s41598-023-34928-9.

33. Adams, R. I.; Miletto, M.; Taylor, J. W.; Bruns, T. D., ‘The diversity and distribution of fungi on residential surfaces’, PLoS ONE 8(11) (2013) e78866, https://doi.org/10.1371/journal.pone.0078866.

34. Méndez-Puentes, C. A.; Camacho-Suárez, J. G.; Echeverry-Hernández, S., ‘Identification of bacteria and fungi in the air of Neiva, Colombia’, Revista de Salud Pública 17(5) (2015) 728-737, https://doi.org/10.15446/rsap.v17n5.38468.

35. Zaragoza, O., Los hongos microscópicos, ¿Amigos o enemigos?, Instituto de Salud Carlos III (ISCIII), Centro Nacional de Microbiología (CNM), Madrid (2018), https://repisalud.isciii.es/handle/20.500.12105/11824.

36. Arenas Guzmán, R., Micología médica ilustrada, 6ª ed., McGraw-Hill Interamericana, Ciudad de México (2019).

37. Bonifaz Trujillo, J. A., Micología médica básica, 6ª ed., McGraw Hill, Ciudad de México (2020).

38. Senthilkumar, M.; Anandham, R.; Krishnamoorthy, R., ‘Paecilomyces’, in Beneficial microbes in agro-ecology, eds. N. Amaresan, M. S. Kumar, K. Annapurna, K. Kumar & A. Sankaranarayanan, Elsevier, London (2020) 793-808, https://doi.org/10.1016/B978-0-12-823414-3.00041-1.

Published

2026-06-03

How to Cite

Rodríguez Sáenz, A. Y., Meléndez Álvarez, B., & Argüello García, P. M. (2026). Diversity and potential pathogenicity of microscopic fungi in the Archaeological Museum Park of Tunja at the Pedagogical and Technological University of Colombia. Conservar Património, 53, 102–113. https://doi.org/10.14568/cp39647