Fungal stains on paper: is what you see what you get?

Sílvia Oliveira Sequeira; Hugo Paiva de Carvalho; Nuno Mesquita; António Portugal; Maria Filomena Macedo

doi:10.14568/cp2018007

Autores

Sílvia Oliveira Sequeira Departamento de Conservação e Restauro, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal; VICARTE, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal https://orcid.org/0000-0002-4882-7133
Hugo Paiva de Carvalho Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal https://orcid.org/0000-0002-7234-0173
Nuno Mesquita Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal https://orcid.org/0000-0002-1748-6364
António Portugal Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal https://orcid.org/0000-0003-1748-6345
Maria Filomena Macedo Departamento de Conservação e Restauro, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal; VICARTE, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal https://orcid.org/0000-0003-4366-4192

DOI:

https://doi.org/10.14568/cp2018007

Palavras-chave:

Manchas de fungos, Conservação de papel, Identificação de fungos, SEM, ADN

Resumo

A mitigação da biodeterioração causada por fungos em obras de arte e documentos em papel representa um desafio para os conservadores em todo o mundo. Inúmeras listas de espécies fúngicas têm sido identificadas a partir de coleções em papel, mas serão estas espécies responsáveis pelos respetivos fenómenos de biodeterioração? O presente estudo teve como objetivo associar espécies de fungos específicas a manchas observadas em documentos e obras de arte em papel. Foram amostradas 23 manchas existentes em três obras em papel. A observação in situ das estruturas fúngicas, com recurso a microscópio ótico e microscópio eletrónico de varrimento, foi comparada com a identificação de isolados através de técnicas de biologia molecular. As manchas negras e de tom castanho-escuro foram associadas às espécies Chaetomium globosum, C. murorum, Penicillium chrysogenum, P. commune, Myxotrichum deflexum e Stachybotrys chartarum. A espécie Eurotium rubrum foi isolada de uma mancha de foxing e a espécie Penicillium citrinum foi identificada em manchas de tom laranja claro.

Recebido: 2018-3-7
Revisto: 2018-9-21
Aceite: 2018-10-12
Online: 2018-11-16
Publicação: 2019-11-6

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