Evaluation of the antifungal efficiency of biocides currently applied in the Coimbra UNESCO area limestone monuments

João Trovão; António Portugal

doi:10.14568/cp25076

Authors

João Trovão Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal https://orcid.org/0000-0002-7845-6046
António Portugal Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal; Fitolab-Laboratory for Phytopathology, Instituto Pedro Nunes, 3030-199 Coimbra, Portugal https://orcid.org/0000-0003-1748-6345

DOI:

https://doi.org/10.14568/cp25076

Keywords:

Biodeterioration, Biotin T, Fungi, O. basilicum essential oil, Limestones, Preventol R80

Abstract

The application of biocides to control microbial proliferation in stone materials is nowadays a rather common practice. In the Coimbra´s UNESCO World heritage monuments, biocide treatments rely on the application of Biotin T and Preventol R80. However, the application of these formulations often occurs without the complete knowledge of microbial communities’ susceptibility to their application. The aim of this study was to evaluate the efficiency of these compounds and compare them with a commercial version of Ocimum basilicum essential oil, against various fungal species present in these areas. Through the application of in vitro antifungal activity assays, we were able to verify that, in general, Biotin T rather than Preventol R80 was more efficient against all tested fungi. In addition, the commercial version of the Ocimum basilicum essential oil also exhibited good results and might be an alternative option to be taken into consideration in future interventions in these monuments.

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References

Pinna, D., Coping with biological growth on stone heritage objects: methods, products, applications, and perspectives, AAP Apple Academic Press, Oakville (2017).

Cappitelli, F.; Cattò, C.; Villa, F., ‘The Control of Cultural Heritage Microbial Deterioration’, Microorganisms 8 (2020) 1542, https://doi.org/10.3390/microorganisms8101542.

Lo Schiavo, S.; De Leo, F.; Urzì, C., ‘Present and Future Perspectives for Biocides and Antifouling Products for Stone-Built Cultural Heritage: Ionic Liquids as a Challenging Alternative’, Applied Sciences 10 (2020) 6568, https://doi.org/10.3390/app10186568.

Sanmartín, P.; Carballeira, R., ‘Changes in heterotrophic microbial communities induced by biocidal treatments in the Monastery of San Martiño Pinario (Santiago de Compostela, NW Spain)’, International Biodeterioration and Biodegradation 156 (2021) 105130, https://doi.org/10.1016/j.ibiod.2020.105130.

Fidanza, M. R.; Caneva, G., ‘Natural biocides for the conservation of stone cultural heritage: A review’, Journal of Cultural Heritage 38 (2019) 271-286, https://doi.org/10.1016/j.culher.2019.01.005.

Marco, A.; Santos, S.; Caetano, J.; Pintado, M.; Vieira, E.; Moreira, P. R., ‘Basil essential oil as an alternative to commercial biocides against fungi associated with black stains in mural painting’, Building and Environment 167 (2020) 106459, https://doi.org/10.1016/j.buildenv.2019.106459.

Scheerer, S., Microbial Biodeterioration of Outdoor Stone Monuments. Assessment Methods and Control Strategies, Cardiff University, Cardiff (2008).

Doehne, E.; Price, C. A., Stone Conservation: An Overview of Current Research, 2nd ed., The Getty Conservation Institute, Los Angeles (2010).

Sterflinger, K., ‘Fungi: Their role in deterioration of cultural heritage’, Fungal Biology Reviews 24 (2010) 47-55, https://doi.org/10.1016/j.fbr.2010.03.003.

Trovão, J.; Portugal, A.; Soares, F.; Paiva, D. S.; Mesquita, N.; Coelho, C.; Pinheiro, A.C.; Catarino, L.; Gil, F.; Tiago, I., ‘Fungal diversity and distribution across distinct biodeterioration phenomena in limestone walls of the old cathedral of Coimbra, UNESCO World Heritage Site’, International Biodeterioration & Biodegradation 142 (2019) 91-102, https://doi.org/10.1016/j.ibiod.2019.05.008.

Trovão, J.; Gil, F.; Catarino, L.; Soares, F.; Tiago, I.; Portugal, A., ‘Analysis of fungal deterioration phenomena in the first Portuguese King tomb using a multi– analytical approach’, International Biodeterioration & Biodegradation 149 (2020) 104933, https://doi.org/10.1016/j.ibiod.2020.104933.

Trovão, J.; Tiago, I.; Catarino, L.; Gil, F.; Portugal, A., ‘In vitro analyses of fungi and dolomitic limestone interactions: bioreceptivity and biodeterioration assessment’, International Biodeterioration & Biodegradation 155 (2020) 105107, https://doi.org/10.1016/j.ibiod.2020.105107.

Trovão, J.; Soares, F.; Tiago, I.; Catarino, L.; Portugal, A.; Gil, F., ‘A contribution to understand the Portuguese emblematic Ançã limestone bioreceptivity to fungal colonization and biodeterioration’, Journal of Cultural Heritage 49 (2021) 305-312, https://doi.org/10.1016/j.culher.2021.04.003.

Bauer, A.; Perry, D. M.; Kirby, W. M. M., ‘Single disc antibiotic sensitivity testing of Staphylococci’, A.M.A. Archives of Internal Medicine 104 (1959) 208-216, https://doi.org/10.1001/archinte.1959.00270080034004.

Favero-Longo, S. E.; Vannini, A.; Benesperi, R.; Bianchi, E.; Fačkovcová, Z.; Giordani, P.; Malaspina, P.; Martire, L.; Matteucci, E.; Paoli, L.; Ravera, S.; Roccardi, A.; Tonon, C.; Loppi, S., ‘The application protocol impacts the effectiveness of biocides against lichens’, International Biodeterioration and Biodegradation 155 (2020) 105105, https://doi.org/10.1016/j.ibiod.2020.105105.

Li, T.; Hu, Y.; Zhang, B., ‘Evaluation of efficiency of six biocides against microorganisms commonly found on Feilaifeng Limestone, China’, Journal of Cultural Heritage 43 (2020) 45-50, https://doi.org/10.1016/j.culher.2019.11.006.

Mateus, D. M. R.; Silva, R. B.; Costa, F. M. C.; Coroado, J. P. F., ‘Microbiological diversity in the Unfinished Sacristy building of the Convent of Christ, Tomar, and evaluation of its biocide-based control’, Conservar Património 17 (2013) 11-20, https://doi.org/10.14568/cp2012005.

de Los Ríos, A.; Cámara, B.; García Del Cura, M. A. A.; Rico, V. J.; Galván, V.; Ascaso, C., ‘Deteriorating effects of lichen and microbial colonization of carbonate building rocks in the Romanesque churches of Segovia, Spain’, Science of the Total Environment 407 (2009) 1123-1134, https://doi.org/10.1016/j.scitotenv.2008.09.042.

Coutinho, M. L.; Miller, A. Z.; Martin‐Sanchez, P. M.; Mirão, J.; Gomez‐Bolea, A.; Machado‐Moreira, B.; Cerqueira‐Alves, L.; Jurado, V.; Saiz‐Jimenez, C.; Lima, A.; Phillips, A. J. L.; Pina, F.; Macedo, M. F., ‘A multiproxy approach to evaluate biocidal treatments on biodeteriorated majolica glazed tiles’, Environmental Microbiology 18 (2016) 4794-4816, https://doi.org/10.1111/1462-2920.13380.

Vannini, A.; Contardo, T.; Paoli, L.; Scattoni, M.; Favero-Longo, S. E.; Loppi, S., ‘Application of commercial biocides to lichens: Does a physiological recovery occur over time?’, International Biodeterioration and Biodegradation 129 (2018) 189-194, https://doi.org/10.1016/j.ibiod.2018.02.010.

Blazquez, A. B.; Lorenzo, J.; Flores, M.; Gómez-Alarcón, G., ‘Evaluation of the effect of some biocides against organisms isolated from historic monuments’, Aerobiologia 16 (2000) 423-428, https://doi.org/10.1023/A:1026562407821.

Monte, M.; Ferrari, R.; Lonati, G.; Malagodi, M., ‘Biocidal Activity on Microbic Bio- Deteriogens and on Frescoes’, in Science and Technology for the Safeguard of Cultural Heritage in the Mediterranean Basin, Elsevier, Paris (2000), 633-639.

Fierascu, I.; Ion, R.-M.; Radu, M.; Dima, Ş.-O.; Suica-Bunghez, I.-R.; Avramescu, S.; Fierascu, R., ‘Comparative study of antifungal effect of natural extracts and essential oils of Ocimum basilicum on selected artefacts’, Revue Roumaine de Chimie 59 (2014) 207-211.

Hussain, A.I.; Anwar, F.; Hussain Sherazi, S.T.; Przybylski, R., ‘Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations’, Food Chemistry 108 (2008) 986-995, https://doi.org/10.1016/j.foodchem.2007.12.010.

Avetisyan, A.; Markosian, A.; Petrosyan, M.; Sahakyan, N.; Babayan, A.; Aloyan, S.; Trchounian, A., ‘Chemical composition and some biological activities of the essential oils from basil Ocimum different cultivars’, BMC Complementary Medicine and Therapies 17 (2017) 60, https://doi.org/10.1186/s12906-017-1587-5.

Khan, A.; Ahmad, A.; Akhtar, F.; Yousuf, S.; Xess, I.; Khan, L. A.; Manzoor, N., ‘Induction of oxidative stress as a possible mechanism of the antifungal action of three phenylpropanoids’, FEMS Yeast Research 11 (2011) 114-122, https://doi.org/10.1111/j.1567-1364.2010.00697.x.

Khan, A.; Ahmad, A.; Akhtar, F.; Yousuf, S.; Xess, I.; Khan, L. A.; Manzoor, N., ‘Ocimum sanctum essential oil and its active principles exert their antifungal activity by disrupting ergosterol biosynthesis and membrane integrity’, Research in Microbiology 161 (2010) 816-823, https://doi.org/10.1016/j.resmic.2010.09.008.

Zuena, M.; Ruggiero, L.; Caneva, G.; Bartoli, F.; Ventura, G. D.; Ricci, M. A.; Sodo, A., ‘Assessment of Stone Protective Coatings with a Novel Eco-Friendly Encapsulated Biocide’, Coatings 11 (2021) 1109, https://doi.org/10.3390/coatings11091109.

Salvadori, O.; Charola, A. E., ‘Methods to Prevent Biocolonization and Recolonization: An Overview of Current Research for Architectural and Archaeological Heritage’, in Biocolonization of Stone: Control and Preventive Methods: Proceedings from the MCI Workshop Series, eds. A. Elena Charola, Christopher McNamara and Robert J. Koestler, Smithsonian Institution Scholarly Press, Washington (2011) 37-50.